Acclimatization of secondary somatic embryos derived plants of Eucalyptus globulus Labill.: an ultrastructural approach

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Pinto, G (Author) Other; Silva, S (Author) Other; Loureiro, J (Author) Other; Costa, A (Author) Other; Dias, MC (Author) Other; Araujo, C (Author) Other; Neves, L (Author) Other; Santos, C (Author) Other

Journal

Title: Trees - Structure and Function

Vol. 25

Pages: 383-392

ISSN: 0931-1890

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 17 Citations

Other information

Authenticus ID: P-002-R32

DOI: 10.1007/s00468-010-0513-y

Abstract (EN): This paper reports the complete process from secondary emblings (SE-derived plants) regeneration to acclimatization of Eucalyptus globulus and describes histocytological changes that occur in leaves from in vitro to ex vitro acclimatization for a 3-month period. After elongation, plants were transferred to pots with sterilized peat:perlite and acclimatized in a phytotron, with progressive reduction of RH and increase of light intensity. Histocytological analyses were performed in fixed material using light microscopy and ultrastructural changes followed by electron microscopy (SEM and TEM). The protocol used allowed the successful acclimatization of the emblings. Plants looked morphologically normal and FCM screening revealed no ploidy or DNA content abnormalities. Histocytological analyses showed significant changes along time, mostly in stomata shape and aperture, starch reserves, chloroplast morphology and mesophyll differentiation. This is the first report concerning emblings acclimatization to ex vitro conditions in Eucalyptus. It was clearly demonstrated that during acclimatization emblings suffered profound changes in leaf morphology in order to successfully adapt to ex vitro conditions.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10