Detection of somaclonal variants in somatic embryogenesis-regenerated plants of Vitis vinifera by flow cytometry and microsatellite markers

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Jesus Prado, MJ (Author) Other; Rodriguez, E (Author) Other; Rey, L (Author) Other; Victoria Gonzalez, MV (Author) Other; Santos, C (Author) Other; Rey, M (Author) Other

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 103

Pages: 49-59

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 51 Citations

Scopus - 53 Citations

Other information

Authenticus ID: P-003-2MV

DOI: 10.1007/s11240-010-9753-1

Abstract (EN): Flow cytometry and microsatellite analyses were used to evaluate the trueness-to-type of somatic embryogenesis-regenerated plants from six important Spanish grapevine (Vitis vinifera L.) cultivars. Tetraploid plants were regenerated through somatic embryogenesis from all of the cultivars tested with the exception of 'Merenzao'. In addition, an octoploid plant was obtained in the cv. 'Albario', and two mixoploids in 'Torront,s'. The most probable origin of these ploidy variations is somaclonal variation. The cv. 'Brancellao' presented significantly more polyploids (28.57%) than any other cultivar, but it must be noted that 50% of the adult field-grown 'Brancellao' mother plants analysed were mixoploid. Hence, it is probable that these polyploids originated either from somaclonal variation or by separation of genotypically different cell layers through somatic embryogenesis. Microsatellite analysis of somatic embryogenesis-regenerated plants showed true-to-type varietal genotypes for all plants except six 'Torront,s' plants, which showed a mutant allele (231) instead of the normal one (237) at the locus VVMD5. There was not a clear relationship between the occurrence of the observed mutant regenerated plants and the callus induction media composition, the developmental stage of the inflorescences, the type of explant used for starting the cultures or the type of germination (precocious in differentiation medium or normal in germination medium) in any of the cultivars tested, except 'Torront,s'. The mutant plants described herein have been transplanted to soil for future evaluation of putative phenotypic traits of interest. These mutants can be useful both for breeding programs and for functional genomic approaches aimed at increasing knowledge of the biology of grapevine.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11