Determination of glucose in soft drink and sugar-cane juice employing a multicommutation approach in flow system and enzymatic reaction

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Kronka, EAM (Author) Other; Paim, APS (Author) Other; Reis, BF (Author) Other; Lima, JLFC (Author) FFUP; Lapa, RAS (Author) FFUP

Journal

Title: Fresenius' Journal of Analytical Chemistry

Vol. 364

Pages: 358-361

ISSN: 0937-0633

Publisher: Springer

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-001-466

DOI: 10.1007/s002160051349

Abstract (EN): A flow system based on a multicommutation approach was developed for the determination of glucose and sucrose employing enzymatic reactions. The determination was based on the reaction with D-glucose generating hydrogen peroxide catalyzed by glucose-oxidase (GOD). Subsequently, the H2O2 generated reacts with 4-aminefenazone plus phenol to form 4-(p-benzoquinone-monoimine) fenazone detected at 510 nm. This reaction is catalyzed by the peroxidase enzyme (POD). The flow network comprised a set of three-way solenoid valves and was controlled by means of a microcomputer furnished with an electronic interface and running a software written in Quick BASIC 4.5. The flow network and control software were designed to implement the multicommutation approach providing facilities to handle sample and reagent solutions, so that, sample dilution could be easily performed on line. Accuracy was assessed by comparison with results obtained by known procedures and no significant difference at the 95% confidence level was observed. Other advantageous features were a linear response ranging from 0.05 to 0.20% (w/v) glucose without prior dilution, a reagent consumption of 336 mu L per determination, an analytical throughput of 30 samples per hour.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4