Heterologous protein production in Escherichia coli biofilms: a non-conventional form of high cell density cultivation

• Type: Article in International Scientific Journal
• Date: 2017

Authors

L. C. Gomes (Author) FEUP; F. J. Mergulhão (Author) FEUP

Journal

Title: Process Biochemistry

Vol. 57

Pages: 1-8

ISSN: 1359-5113

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 3 Citations

ISI Web of Science

Scopus - 6 Citations

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

Other information

Authenticus ID: P-00N-68N

DOI: 10.1016/j.procbio.2017.03.018

Abstract (EN): Escherichia coli is one of the favourite hosts for recombinant protein production and is recognized as an excellent model for biofilm studies. High cell density cultures (HCDC) of this bacterium enable attractive volumetric production yields and cells growing in biofilms share some of the challenges of conventional high cell density planktonic cultures. This work assesses the production potential of E. coli JM109(DE3) biofilm cells expressing a model protein, the enhanced green fluorescent protein (eGFP), from a recombinant plasmid. A control strain harbouring the same plasmid backbone but lacking the eGFP gene was used to assess the impact of heterologous protein production on biofilm formation. Results show that specific eGFP production from biofilm cells was about 30 fold higher than in planktonic state. Moreover, eGFP-expressing cells had enhanced biofilm formation compared to control cells. Volumetric production values were 2 fold higher than those previously reported with the same protein and are within the range of what can be obtained by conventional HCDC in the production of soluble proteins. Although the cellular density that was obtained was lower than in conventional HCDC (0.5 fold), this novel system reached good production values which are likely to be improved after optimization of culture conditions.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8