Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: in vitro mineralization and biological evaluation

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Filipa Castro (Author) FEUP; António Ferreira (Author) FEUP; Viviana P. Ribeiro (Author) Other; Ana L. Oliveira (Author) Other; Rui L. Reis (Author) Other; José A. Teixeira (Author) Other; Fernando Rocha (Author) FEUP

Journal

Title: Materials Research Express

Vol. 3 No. 7

Final page: 075404

Publisher: Institute of Physics Publishing

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 8 Citations

INSPEC

Scientific classification

CORDIS: Technological sciences > Engineering > Chemical engineering

FOS: Engineering and technology > Chemical engineering

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

IF/01087/2014/CP1249/CT0001 - Projeto de investigação exploratória

Other information

Authenticus ID: P-00K-R6C

DOI: 10.1088/2053-1591/3/7/075404

Abstract (EN): This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physicochemical characterization of the synthesized HAp suggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 mu g ml(-1)), although its cellular uptake behavior. Therefore, the prepared HAp shows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13