Long-term stability of endogenous B-type natriuretic peptide during storage at -20 degrees C for later measurement with Blosite Triage assay

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Pereira, M (Author) Other; Azevedo A (Author) FMUP; Severo M (Author) FMUP; Barros H (Author) FMUP

Journal

Title: Clinical Biochemistry

Vol. 40

Pages: 1104-1107

ISSN: 0009-9120

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-004-6YC

DOI: 10.1016/j.clinbiochem.2007.06.014

Abstract (EN): objective: To evaluate the Ion.-term stability of B-type natriuretic peptide (BNP) in frozen samples at -20 degrees C. Design and methods: During a health and nutrition survey of a representative sample of the adult population of Porto, Portugal, blood samples were collected in EDTA tubes. Aliquots of plasma were frozen at -20 degrees C until measurement. Subjects with cardiac structural or functional abnormialities or renal dysfunction were excluded. BNP was assessed using a commercial immunofluorescence assay (Triage BNP Test((R))) as a continuous variable (n=340) and in four subgroups selected according to different storage periods: :! I month (n=35), 6-12 months (n=80), 2024 months (n=18) and >= 36 months (n=21). Results: Age- and sex-adjusted BNP values, analyzed as a continuous variable, decreased significantly over time of storage. When we stratified in four intervals of storage time, the median BNP value (25th-75th percentile, p value) was: 22.6 pg/mL (8.9-76.2, reference group) for <= 1 month, 19.8 pg/mL (9.5-48.4, p=0.055) for 6-12 months, 15.3 pg/mL (7.5-35.0, p=0.037) for 20-24 months, and 2.5 pg/mL (2.5-10.5, p < 0.001) for >= 36 months. Conclusions: BNP is stable at -20 degrees C for 1 year, without protease inhibitors.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4