Adsorbed fibrinogen leads to improved bone regeneration and correlates with differences in the systemic immune response

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Santos, SG (Author) Other; M Lamghari (Author) ICBAS; Almeida, CR (Author) Other; Oliveira, MI (Author) ICBAS; Neves, N (Author) FMUP; Ribeiro, AC (Author) Other; Judite N Barbosa (Author) ICBAS; Barros, R (Author) Other; Maciel, J (Author) Other; Martins, MCL (Author) Other; Goncalves, RM (Author) ICBAS; Mário Adolfo Barbosa (Author) ICBAS

Journal

Title: Acta Biomaterialia

Vol. 9

Pages: 7209-7217

ISSN: 1742-7061

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 45 Citations

Scopus - 49 Citations

Other information

Authenticus ID: P-006-9HH

DOI: 10.1016/j.actbio.2013.04.008

Abstract (EN): Designing new biomaterials that can modulate the inflammatory response instead of attempting just to reduce it constitutes a paradigm change in regenerative medicine. This work aimed to investigate the capacity of an immunomodulatory biomaterial to enhance bone regeneration. For that purpose we incorporated a molecule with well-established pro-inflammatory and pro-healing roles, fibrinogen, in chitosan scaffolds. Two different incorporation strategies were tested, leading to concentrations of 0.54 +/- 0.10 mg fibrinogen g(-1) scaffold immediately upon adsorption (Fg-Sol), and 0.34 +/- 0.04 mg fibrinogen g(-1) scaffold after washing (Fg-Ads). These materials were implanted in a critical size bone defect in rats. At two months post-implantation the extent of bone regeneration was examined by histology and the systemic immune response triggered was evaluated by determining the percentages of myeloid cells, T and B lymphocytes in the draining lymph nodes. The results obtained indicate that the fibrinogen incorporation strategy conditioned the osteogenic capacity of biomaterials. Fg-Ads scaffolds led to more bone formation, and the presence of Fg stimulated angiogenesis. Furthermore, animals implanted with Fg-Ads scaffolds showed significant increases in the percentages of B lymphocytes and myeloid cells in the draining lymph nodes, while levels of T lymphocytes were not significantly different. Finally, a significant increase in TGF-beta was detected in the plasma of animals implanted with Fg-Ads. Taken together the results presented suggest a potential correlation between the elicited immune response and biomaterial osteogenic performance.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9