The influence of functional groups of self-assembled monolayers on fibrous capsule formation and cell recruitment

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Judite N Barbosa (Author) Other; Madureira, P (Author) Other; Mário Adolfo Barbosa (Author) FEUP; AP Águas (Author) ICBAS

Journal

Title: Journal of Biomedical Materials Research - Part A

Vol. 76A

Pages: 737-743

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 44 Citations

Other information

Authenticus ID: P-004-MR0

DOI: 10.1002/jbm.a.30602

Abstract (EN): The contribution of the Surface chemistry of an implant to the thickness of the fibrous capsule formed after implantation was herein investigated. For that, self-assembled monolayers (SAMs) of alkanethiols oil gold with different terminal functional groups (COOH, OH, and CH.) were used. These surfaces were implanted ill Subcutaneous air pouches of BALB/c mice and the ensuing fibrous capsules were evaluated and compared with the initial inflammatory response caused by the implant. The thickness of the fibrous capsules that are under organization around the implant was measured I week after implantation by histology. Inflammatory exudates were collected from the air Pouches 24 h after the implantation of SAMs and were analyzed by flow cytometry. A significant increase in the thickness of fibrous capsules was seen around implanted CH3-terminated SAMs, and also in gold surfaces, in comparison with the air pouch wall of sham-operated mice and of COOH- and OH-covered SAMs. The CH3-coated implants also recruited higher numbers of inflammatory cells; this enhancement involved a significant number of Mac-1(+) cells. Our data indicate that implant surfaces coated with CH, induce thick fibrous capsules and this may be the result of the stronger inflammatory effect of CH, in comparison with COOH or OH chemical groups. (c) 2005 Wiley Periodicals, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7