Quantitative MUC5AC and MUC6 mucin estimations in gastric mucus by a least-squares minimization method

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Squire, JM (Author) Other; Guerreiro, MJ (Author) Other; Sidebotham, RL (Author) Other; Celso Reis (Author) ICBAS; Wiseman, J (Author) Other; Luther, PK (Author) Other

Journal

Title: Analytical Biochemistry

Vol. 439

Pages: 204-211

ISSN: 0003-2697

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-006-87C

DOI: 10.1016/j.ab.2013.04.013

Abstract (EN): We have determined the molar proportions of the MUC5AC and MUC6 mucus glycoproteins (mucins) in mucus from the normal and pathological human gastric antrum using a least-squares minimization analysis applied to amino acid compositions. We noted that the content of MUC5AC mucin in mucus from individuals without gastroduodenal disease was very high, suggesting that the integrity and barrier properties of the adherent gastric mucus layer are normally maintained by building-block structures formed from this mucin alone. We observed that the molar content of MUC6 mucin doubled (without significance) in mucus from patients with duodenal ulcer, and increased five times (with high significance) in mucus from patients with gastric ulcer, when compared with that in mucus from individuals without gastroduodenal disease.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8