Sialyl Lewis(x)-dependent binding of human monocyte-derived dendritic cells to selectins

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Silva, Z (Author) Other; Tong, ZQ (Author) Other; Guadalupe Cabral, MG (Author) Other; Martins, C (Author) Other; Castro, R (Author) Other; Celso Reis (Author) ICBAS; Trindade, H (Author) Other; Konstantopoulos, K (Author) Other; Videira, PA (Author) Other

Journal

Title: Biochemical and Biophysical Research Communications

Vol. 409

Pages: 459-464

ISSN: 0006-291X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 19 Citations

Scopus - 19 Citations

Other information

Authenticus ID: P-002-QGV

DOI: 10.1016/j.bbrc.2011.05.026

Abstract (EN): The limited efficacy of monocyte-derived dendritic cell (mo-DC)-based vaccines is primarily attributed to the reduced mo-DC migratory capacity. One undefined aspect is the initial binding of mo-DCs to endothelial cells and vascular selectins. In this study, we investigated the role and modulation of the selectin binding determinant sialyl Lewis(x) (sLe(x)) in selectin-dependent mo-DC binding. Our data reveal that sLe(x) is required for maximal binding of mo-DCs to tumor necrosis factor (TNF)-alpha-activated endothelial cells under static conditions, as evidenced by the use of sialidase. Sialidase treatment also abrogated mo-DC cell tethering to immobilized, purified P-, L-, or E-selectin under flow. The requirement of sLe(x)-dependent binding of mo-DC to selectins was further substantiated by using sLe(x) free sugar and anti-sLe(x) antibody, which significantly suppressed mo-DC-selectin binding. P-selectin glycoprotein ligand-1 is required for mo-DC binding to both P- and L-selectin, but it is dispensable for E-selectin recognition. Interestingly, the extent of mo-DC tethering was maximal on P-selectin, followed by E- and L- selectin. Accordingly, L-selectin mediated faster mo-DC rolling than E- or P-selectin. Interferon (IFN)-gamma induces a significant increase in mo-DC surface sLe(x\) expression, which is probably due to the enhanced synthesis of C2GnT-I. These findings may contribute to improving mo-DC-based vaccination protocols.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6