Different expression levels of alpha 3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Escrevente, C (Author) Other; Machado, E (Author) Other; Brito, C (Author) Other; Celso Reis (Author) FMUP; Stoeck, A (Author) Other; Runz, S (Author) Other; Marme, A (Author) Other; Altevogt, P (Author) Other; Costa, J (Author) Other

Journal

Title: International Journal of Oncology

Vol. 29

Pages: 557-566

ISSN: 1019-6439

Publisher: Demetrios A. Spandidos Ed. & Pub.

Indexing

ISI Web of Knowledge - 49 Citations

Scopus - 50 Citations

Other information

Authenticus ID: P-004-HJ1

Abstract (EN): Ovarian carcinoma is the leading cause of death from gynecological cancers in many countries. Fucosylated glycoconjugates have been associated with various carcinomas. In the present study, we have characterized the expression of alpha 3/4 fucosyltransferases transcripts and their products, the Lewis carbohydrate determinants, and their in vitro specificity towards synthetic acceptors using ovarian carcinoma cell lines OVM, m130, GG and SKOV3. We found different expression patterns: GG cells expressed mostly Lewis(x) (Le(x)), Lewis(y) (Le(y)), sLe(a) and Le(b), and m130 cells expressed mostly Le(x) and Le(y). The detection was on the plasma membrane and in intracellular vesicles. OVM and SKOV3 cells had very low amounts of staining. From RT-PCR studies, enzyme specificity of cellular extracts towards a panel of synthetic carbohydrate acceptors and Western blot analysis we concluded that Le(a), sLe(a) and Le(b) were synthesised by FUT3, whereas Le(x) and Le(y) were synthesized by FUT4 and FUT9 in both cell lines. The GG and m130 cell lines are adequate models to investigate the role of Le(x), Le(y), sLe(a) and Le(b) in ovarian carcinoma development.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10