Comparison between optical measurements made from natural and frozen samples at optical clearing

• Type: Article in International Conference Proceedings Book
• Date: 2013

Authors

Oliveira, L (Author) Other; Maria Inês Barbosa de Carvalho (Author) FEUP; Nogueira, E (Author) Other; Tuchin, VV (Author) Other

Conference proceedings International

Title: SARATOV FALL MEETING 2012: OPTICAL TECHNOLOGIES IN BIOPHYSICS AND MEDICINE XIV; AND LASER PHYSICS AND PHOTONICS XIV

Saratov Fall Meeting on Optical Technologies in Biophysics and Medicine XIV; and Laser Physics and Photonics XIV

Saratov, RUSSIA, SEP 25-28, 2012

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Other information

Authenticus ID: P-006-FVR

DOI: 10.1117/12.2016936

Abstract (EN): To determine the differences between the optical clearing effects created by ethylene glycol in fresh and frozen samples, we have performed several measurements from samples in both conditions. Fresh samples were used after animal sacrifice and frozen samples were kept at -20 degrees C for 72 hours. The different measurements performed with samples from both cases were total transmittance, collimated transmittance, total reflectance and specular reflectance. Considering, for instance, collimated transmittance measurements, we have verified that the spectra measured from both samples before adding the solution present different levels of collimated transmittance. The time-dependence evolution of the collimated transmittance spectrum is similar between both cases of samples, but since they present different levels of "natural" transmittance, the optical clearing effect is observed at different levels if we compare between fresh and frozen samples.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 18