Diffusion characteristics of ethylene glycol in skeletal muscle

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Oliveira, LM (Author) Other; Maria Inês Barbosa de Carvalho (Author) FEUP; Nogueira, EM (Author) Other; Tuchin, VV (Author) Other

Journal

Title: Journal of Biomedical Optics

Vol. 20

Final page: 051019

ISSN: 1083-3668

Publisher: SPIE

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 22 Citations

Other information

Authenticus ID: P-00A-348

DOI: 10.1117/1.jbo.20.5.051019

Abstract (EN): Part of the optical clearing study in biological tissues concerns the determination of the diffusion characteristics of water and optical clearing agents in the subject tissue. Such information is sufficient to characterize the time dependence of the optical clearing mechanisms-tissue dehydration and refractive index (RI) matching. We have used a simple method based on collimated optical transmittance measurements made from muscle samples under treatment with aqueous solutions containing different concentrations of ethylene glycol (EG), to determine the diffusion time values of water and EG in skeletal muscle. By representing the estimated mean diffusion time values from each treatment as a function of agent concentration in solution, we could identify the real diffusion times for water and agent. These values allowed for the calculation of the correspondent diffusion coefficients for those fluids. With these results, we have demonstrated that the dehydration mechanism is the one that dominates optical clearing in the first minute of treatment, while the RI matching takes over the optical clearing operations after that and remains for a longer time of treatment up to about 10 min, as we could see for EG and thin tissue samples of 0.5 mm. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10