Effect of OnabotulinumtoxinA on Intramural Parasympathetic Ganglia: An Experimental Study in the Guinea Pig Bladder

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Coelho, A (Author) Other; Francisco Cruz (Author) FMUP; Cruz C.D. (Author) FMUP; António Avelino Silva (Author) FMUP

Journal

Title: Journal of Urology

Vol. 187

Pages: 1121-1126

ISSN: 0022-5347

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 23 Citations

Other information

Authenticus ID: P-002-CV5

DOI: 10.1016/j.juro.2011.10.130

Abstract (EN): Purpose: We investigated whether onabotulinumtoxinA injected in the bladder would affect preganglionic parasympathetic nerve endings in intramural ganglia. Materials and Methods: Guinea pig bladders were injected with 5 U of botulinum toxin. At 24 hours bladders were collected and processed for immunohistochemistry using tyrosine hydroxylase, and intact and cleaved SNAP-25. To identify the different populations of affected fibers coursing the ganglia we performed double immunoreactions for cleaved SNAP-25 and VAChT, TH or CGRP. Results: VAChT immunoreactive fibers were identified in axons and varicosities of presynaptic to postganglionic parasympathetic neurons. Those fibers were also immunoreactive to SV2 and SNAP-25. The rare CGRP and TH immunoreactive fibers coursing in the ganglia did not express SV2 or SNAP-25. After onabotulinumtoxinA injection the cleaved form of SNAP-25 was abundantly expressed in parasympathetic fibers. Conclusions: Botulinum toxin injection in the bladder wall affects preganglionic parasympathetic nerve terminals. This could contribute to the strong effect of botulinum toxin on bladder smooth muscle activity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6