Helicobacter pylori infection affects mitochondrial function and DNA repair, thus, mediating genetic instability in gastric cells

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Machado, AMD (Author) Other; Desler, C (Author) Other; Boggild, S (Author) Other; Strickertsson, JAB (Author) Other; Friis Hansen, L (Author) Other; Figueiredo C (Author) FMUP; Seruca, R (Author) FMUP; Rasmussen, LJ (Author) Other

Journal

Title: Mechanisms of Ageing and Development

Vol. 134

Pages: 460-466

ISSN: 0047-6374

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 35 Citations

Other information

Authenticus ID: P-008-HCX

DOI: 10.1016/j.mad.2013.08.004

Abstract (EN): Helicobacter pylori infection is an important factor for the development of atrophic gastritis and gastric carcinogenesis. However, the mechanisms explaining the effects of H. pylori infection are not fully elucidated. H. pylori infection is known to induce genetic instability in both nuclear and mitochondrial DNA of gastric epithelial cells. The mutagenic effect of H. pylori infection on nuclear DNA is known to be a consequence, in part, of a down-regulation of expression and activity of major DNA repair pathways. In this study, we demonstrate that H. pylori infection of gastric adenocarcinoma cells causes mtDNA mutations and a decrease of mtDNA content. Consequently, we show a decrease of respiration coupled ATP turnover and respiratory capacity and accordingly a lower level and activity of complex I of the electron transport chain. We wanted to investigate if the increased mutational load in the mitochondrial genome was caused by down-regulation of mitochondrial DNA repair pathways. We lowered the expression of APE-1 and YB-1, which are believed to be involved in mitochondrial base excision repair and mismatch repair. Our results suggest that both APE-1 and YB-1 are involved in mtDNA repair during H. pylori infection, furthermore, the results demonstrate that multiple DNA repair activities are involved in protecting mtDNA during infection.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7