Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Goncalves, CM (Author) Other; Castro, MAA (Author) Other; Henriques, T (Author) Other; Oliveira, MI (Author) Other; Pinheiro, HC (Author) Other; Oliveira C (Author) FMUP; Sreenu, VB (Author) Other; Evans, EJ (Author) Other; Davis, SJ (Author) Other; Moreira, A (Author) REIT; Alexandre Carmo (Author) ICBAS

Journal

Title: Molecular Immunology

Vol. 46

Pages: 2585-2596

ISSN: 0161-5890

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-003-H8H

DOI: 10.1016/j.molimm.2009.05.006

Abstract (EN): Glycoproteins of the scavenger receptor cysteine-rich (SRCR) superfamily contain one or more protein modules homologous to the membrane-distal domain of macrophage scavenger receptor I. These domains can be found in the extracellular regions of membrane proteins and in secreted glycoproteins, from the most primitive species to vertebrates. A systematic, bioinformatics-based search for putative human proteins related to the forty-seven known human group B SRCR domains identified a new family member that we have called Soluble Scavenger with 5 Domains (SSc5D). SSc5D is a new soluble protein whose expression is restricted to monocytes/macrophages and T-lymphocytes, and is particularly enriched in the placenta. The gene encoding SSc5D spans 30 kb of genomic DNA, and contains fourteen exons producing a 4.8 kb-long mRNA. The mature polypeptide is predicted to consist of 1573 amino acids comprising, towards the N-terminus, five very similar SRCR domains that are highly conserved among non-marsupial mammals, and a large (>250 nm), very heavily glycosylated, mucin-like sequence towards the C-terminus. Each of the SRCR domains is encoded by a single exon, and contains eight cysteine residues, as observed for all other group B SRCR domains. A shorter isoform encoded by a weakly expressed, alternatively spliced transcript, which lacks the mucin-like C-terminal region, was also identified. It seems likely that SSc5D has a role at the interface between adaptive and innate immunity, or in placental function.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12