Aurora B and Cyclin B Have Opposite Effects on the Timing of Cytokinesis Abscission in Drosophila Germ Cells and in Vertebrate Somatic Cells

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Mathieu, J (Author) Other; Cauvin, C (Author) Other; Moch, C (Author) Other; Radford, SJ (Author) Other; Sampaio, P (Author) Other; Perdigoto, CN (Author) Other; Schweisguth, F (Author) Other; Bardin, AJ (Author) Other; Sunkel, CE (Author) ICBAS; Mckim, K (Author) Other; Echard, A (Author) Other; Huynh, JR (Author) Other

Journal

Title: Developmental Cell

Vol. 26

Pages: 250-265

ISSN: 1534-5807

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 62 Citations

Scopus - 62 Citations

Other information

Authenticus ID: P-006-88N

DOI: 10.1016/j.devcel.2013.07.005

Abstract (EN): Abscission is the last step of cytokinesis that physically separates the cytoplasm of sister cells. As the final stage of cell division, abscission is poorly characterized during animal development. Here, we show that Aurora B and Survivin regulate the number of germ cells in each Drosophila egg chamber by inhibiting abscission during differentiation. This inhibition is mediated by an Aurora B-dependent phosphorylation of Cyclin B, as a phosphomimic form of Cyclin B rescues premature abscission caused by a loss of function of Aurora B. We show that Cyclin B localizes at the cytokinesis bridge, where it promotes abscission. We propose that mutual inhibitions between Aurora B and Cyclin B regulate the duration of abscission and thereby the number of sister cells in each cyst. Finally, we show that inhibitions of Aurora B and Cyclin-dependent kinase 1 activity in vertebrate cells also have opposite effects on the timing of abscission, suggesting a possible conservation of these mechanisms.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16