In-vitro maturation of sperm

• Type: Article in International Conference Proceedings Book
• Date: 2005

Authors

Sa, R (Author) Other; sousa, m (Author) ICBAS; Cremades, N (Author) Other; Neves, R (Author) Other; Alves, C (Author) Other; Fernandes, S (Author) Other; Silva, J (Author) Other; barros, a (Author) FMUP

Conference proceedings International

Title: Proceedings of the 13th World Congress on In Vitro Fertilization, Assisted Reproduction & Genetics

Pages: 79-82

13th World Congress on In- Vitro Fertilization, Assisted Reproduction and Genetics

Istanbul, TURKEY, MAY 26-29, 2005

Indexing

ISI Web of Knowledge - 0 Citations

Other information

Authenticus ID: P-000-6GD

Abstract (EN): Diploid germ cells (DGC) were isolated and cocultured for 2-weeks in Vero-cell conditioned-medium (CM). FSH and Testosterone (T) elicited 7% of meiosis (new round spermatids, Sa1) and 17% of late spermatid (Sd) differentiation. Developmental arrest occurred at early (Sa1Sa2) and late (Sb-Sd) spermiogenesis. Transmission electron microscopy (TEM), fluorescent in situ hybridisation (FISH) and mRNA expression (RT-PCR) confirmed cell-stage diagnosis and normal meiotic chromosomal synapsis and segregation. DNA replication (BrdU) of DGC was kept for only I week. Cultures were limited by apoptosis, as confirmed by caspase-3 activity, TEM and RT-PCR.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4