Molecular Cytogenetics of Human Single Pronucleated Zygotes

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Azevedo, AR (Author) Other; Pinho, MJ (Author) Other; Silva, J (Author) Other; Sa, R (Author) Other; Thorsteinsdottir, S (Author) Other; barros, a (Author) FMUP; sousa, m (Author) ICBAS

Journal

Title: Reproductive Sciences

Vol. 21

Pages: 1472-1482

ISSN: 1933-7191

Publisher: SAGE

Indexing

ISI Web of Knowledge - 19 Citations

Scopus - 22 Citations

Other information

Authenticus ID: P-009-YV6

DOI: 10.1177/1933719114530185

Abstract (EN): The aim of the present study was to use fluorescence in situ hybridization to analyze the chromosome status of zygotes with a single pronucleus from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatment cycles. In addition, we performed immunocytochemical detection of nuclear lamins and histone H3 trimethylated at lysine-9, Me(3)H3K9. Zygotes were processed 24 hours after insemination or injection to assure the absence of asynchrony. In opposition to previous results, we observed 2 pronuclei in 16 of 18 IVF zygotes and 40 of 64 ICSI zygotes, suggesting premature pronuclear breakdown. In IVF and ICSI zygotes, the rate of normal diploidy was only 6 of 16 and 27 of 56, respectively, suggesting that monopronucleated zygotes should not be used in assisted reproductive treatments. The possible mechanisms are discussed and compared to previous studies of monopronucleated zygotes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11