Effect of in vitro exposure to lead chloride on semen quality and sperm DNA fragmentation

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Gomes, M (Author) Other; Goncalves, A (Author) Other; rocha, e (Author) ICBAS; Sa, R (Author) Other; Alves, A (Author) Other; Silva, J (Author) Other; barros, a (Author) FMUP; Pereira, ML (Author) Other; sousa, m (Author) ICBAS

Journal

Title: Zygote

Vol. 23

Pages: 384-393

ISSN: 0967-1994

Publisher: Cambridge University Press

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00G-2GD

DOI: 10.1017/s0967199413000671

Abstract (EN): Exposure to lead may cause changes in the male reproductive system. We evaluated the effect of lead chloride (PbCl2) in vitro on semen quality from 31 individuals. Samples were incubated at room temperature for two exposure times (4 h and 8 h) and with two concentrations of PbCl2 (15 mu g/ml or 30 mu g/ml). Results showed that PbCl2 significantly inhibited rapid progressive motility and caused an increase in the percentage of tail anomalies in both times and concentrations assessed, as well as a decrease in vitality in the group exposed to 30 mu g/ml PbCl2. A significant increase in immotile sperm was also observed between the group control and the groups submitted to lead. Total motility and DNA fragmentation also showed a significant decrease and increase, respectively, after 4 h of incubation in the group exposed to 30 mu g/ml and in both groups after 8 h of incubation. In conclusion, PbCl2 affected sperm parameters and DNA integrity, which are essential for male fertility.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10