miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Ribeiro, J (Author) Other; Marinho Dias, J (Author) Other; Monteiro, P (Author) Other; Loureiro, J (Author) Other; Baldaque, I (Author) Other; Rui Medeiros (Author) ICBAS; Sousa, H (Author) ICBAS

Journal

Title: BioMed Research International

Vol. 2015 No. ID 531789

Pages: 1-6

ISSN: 2314-6133

Publisher: Hindawi

Indexing

ISI Web of Knowledge - 53 Citations

Scopus - 58 Citations

Other information

Authenticus ID: P-00G-C78

DOI: 10.1155/2015/304584

Abstract (EN): We aimed to characterize miR-125b and miR-34a expression in 114 women with different cervical lesions: normal epithelium with (n = 20) and without (n = 29) HPV infection; LSIL (n = 28); HSIL (n = 29); and ICC (n = 8). miRNA expression analysis was performed by comparing the distinct groups with the reference group (women with normal epithelium without HPV). For miR-125b, we observed a twofold (2(-Delta Delta Ct) = 2.11; P = 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2(-Delta Delta Ct) = 0.21; P = 0.004) in ICC. Similarly, miR-34a expression analysis revealed an increased expression (2(-Delta Delta Ct) = 1.69; P = 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2(-Delta Delta Ct) = 2.08; P = 0.042) in ICC. Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity. Results showed that while miR-34a expression seems to be correlated with invasive cervical cancer, miR-125b expression is significantly changed within the different cervical lesions and their levels should be further investigated as possible predictive/prognostic biomarkers using a noninvasive approach.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6