Flavan-3-ols Transport Across Blood-Brain Barrier

• Type: Other Publications
• Date: 2009

Authors

Ana Faria (Author) FCNAUP; Diogo Pestana (Author) Other; Luis Cruz (Author) Other; Victor De Freitas (Author) FCUP; Isabel Azevedo (Author) Other; Nuno Mateus (Author) FCUP; Conceicao Calhau (Author) FMUP

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-003-M25

DOI: 10.1096/fasebj.23.1_supplement.717.8

Abstract (EN): <jats:sec><jats:label /><jats:p>This work aimed to evaluate the transmembranar transport of flavonoids across RBE¿4 cells (rat capillary cerebral endothelial cells). HPLC¿DAD/MS was used to detect the various phenolic compounds in studied samples (apical and basolateral media), and their metabolites. The metabolites of the tested polyphenols were synthesized to be used as patterns.</jats:p><jats:p>Flavan¿3¿ols passed across RBE¿4 cells (epicatechin>B4 procyanidin>catechin) in a time dependent manner. This transport was stereoselective, involving one or more membrane transporters. Additionally, it was found that the cells were capable to metabolize these compounds, particularly by conjugation with glucuronic acid, since this metabolite was detected. An UDP¿glucuronosyltransferase inhibitor was used (ß¿estradiol) and found to facilitate the transport through the BBB.</jats:p><jats:p>In conclusion: (i) the synthesis of flavonoid metabolites allowed the detection of these compounds; (ii) the studied flavan¿3¿ols were capable of crossing the BBB, possibly reaching the central nervous system, which correlates with some neuroprotective effects described in literature for these class of flavonoids; (iii) these cells have enzymes capable of metabolizing these compounds and to conjugate them with glucuronic acid.</jats:p><jats:p>Financial support from iBeSa and FCT (PTDC/QUI/65501/2006, SFRH/BD/28160/2006 and SFRH/BD/30915/2006) is gratefully acknowledged.</jats:p></jats:sec>

Language: English

Type (Professor's evaluation): Scientific