TRANSIENT BISALBUMINEMIA - SEPARATION BY ISOELECTRIC-FOCUSING OF HUMAN ALBUMIN FRACTIONS LINKED TO DIFFERENT NUMBERS OF BENZYLPENICILLOYL GROUPS

• Type: Article in International Scientific Journal
• Date: 1995

Authors

ROCHA, J (Author) FCUP; BOHNER, J (Author) Other; KOMPF, J (Author) Other

Journal

Title: Electrophoresis

Vol. 16

Pages: 1031-1033

ISSN: 0173-0835

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-GST

DOI: 10.1002/elps.11501601174

Abstract (EN): The behavior of modified human serum albumin (HSA) from penicillin-treated patients and in vitro conjugated samples was studied by isoelectric focusing in the absence and presence of 8 M urea. It is shown that samples classified as bisalbuminemic after separation under native conditions display a spectrum of several major bands with decreasing pIs when focusing is done in the presence of 8 M urea. In this spectrum, the distances between two consecutive major bands are as expected from a 2-unit charge difference. Since this result is compatible with the blockage of a lysine residue with a benzylpenicilloyl (BPO) group, each major band can be interpreted as representing an HSA fraction covalently linked to different numbers of benzylpenicilloyl groups. The separation methods presented therefore seem to constitute simple and reliable tools for a detailed assessment of the extent of BPO covalent binding to HSA both in vivo and in vitro.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3