Sex Steroid Hormones Regulate the Expression of Growth-associated Protein 43, Microtubule-associated Protein 2, Synapsin 1 and Actin in the Ventromedial Nucleus of the Hypothalamus

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Susana I Sa (Author) FMUP; Dulce Madeira, MD (Author) FMUP

Journal

Title: Journal of Molecular Neuroscience

Vol. 46

Pages: 622-630

ISSN: 0895-8696

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-002-CV4

DOI: 10.1007/s12031-011-9650-z

Abstract (EN): The ventromedial nucleus of the hypothalamus is well known for its involvement in the regulation of the female reproductive behavior. The dendritic trees of neurons in its ventrolateral division (VMNvl), the dendritic spines, and the dendritic and spine synapses undergo alterations along the estrous cycle. Because these changes are conspicuous, we thought of interest to examine the influence of sex steroids in the levels of the structural proteins of axons and dendrites. The VMNvl of female rats at all phases of the estrous cycle was labeled for growth-associated protein-43, microtubule-associated protein 2, synapsin 1 and actin. The intensity of the labeling was measured using a modified Brightness-Area-Product method that is sensitive to variations the size of the VMN. The brightness per unit area of these proteins did not undergo significant variations over the estrous cycle, except synapsin 1 that was significantly reduced in diestrus relative to the remaining phases of the ovarian cycle. Conversely, the Brightness-Area-Product of all labeled proteins changed along the estrous cycle and was greater at proestrus than at all other phases. Our results show the presence of estrous cycle-related oscillations in the levels of the structural proteins that are involved in dendritic and synaptic plasticity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9