Effects of ozone in Plantago lanceolata and Salix atrocinerea pollen

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Ferreira, B (Author) Other; Ribeiro, H (Author) Other; Pereira, MS (Author) FCUP; Cruz, A (Author) Other; Abreu, I (Author) Other

Journal

Title: Aerobiologia

Vol. 32

Pages: 421-430

ISSN: 0393-5965

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-00K-03K

DOI: 10.1007/s10453-015-9415-1

Abstract (EN): Pollen can be modified by the increasing environmental pollution which can lead to a rising trend in pollen-induced respiratory allergies. The aim of this work was to study the fertility, protein profile and allergenicity of Plantago lanceolata and Salix atrocinerea pollen after exposure to ozone. The pollen collected directly from the anthers of both species was exposed to O-3 in an environmental chamber, for 6 h at different concentrations. After exposing the pollen, viability tests and in vitro germination were performed. Total protein content was determined by the Bradford method, the polypeptides were separated by SDS-PAGE and the antigenic properties were analyzed by immunological techniques using patients' sera. The concentrations of O-3 tested affected the pollen fertility and the total protein content, but the protein profiles did not show any differences. Furthermore, the immunological tests revealed an increase in IgE binding reactivity when pollen was exposed to the pollutant.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10