Role of physical exercise on hepatic insulin, glucocorticoid and inflammatory signaling pathways in an animal model of non-alcoholic steatohepatitis

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Passos, E (Author) Other; Pereira, CD (Author) Other; Goncalves, IO (Author) Other; Rocha Rodrigues, S (Author) Other; Silva, N (Author) Other; Guimares, JT (Author) Other; Neves, D (Author) Other; António Alexandre Moreira Ribeiro de Ascensão (Author) FADEUP; J. Magalhães (Author) FADEUP; Martins, M. J. (Author) FMUP

Journal

Title: Life Sciences

No. 123

Pages: 51-60

ISSN: 0024-3205

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 18 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Authenticus ID: P-00A-42T

DOI: 10.1016/j.lfs.2014.12.013

Abstract (EN): Aims: Pro-inflammatory mediators, glucocorticoids and transforming growth factor (TGF)-beta are implicated in the pathogenesis of non-alcoholic steatohepatitis (NASH)-related insulin resistance. As physical activity is beneficial against NASH, we analyzed the voluntary physical activity (VPA) and endurance training (ET) (preventive and therapeutic strategies) effects on hepatic insulin, pro-inflammatory and glucocorticoid signaling regulators/mediators in high-fat (Lieber-DeCarli) diet (HFD)-induced NASH. Main methods: Adult male Sprague-Dawley rats were divided in standard diet (SD) or HFD, with sedentary, VPA and ET animals in both diet regimens. Plasma glucose and insulin concentrations were analyzed; plasma insulin sensitivity index (ISI) was calculated. Hepatic insulin, pro-inflammatory and glucocorticoid signaling regulators/mediators were evaluated by Western blot or reverse transcriptase-PCR. Key findings: ET improved ISI in both diet regimens. HFD-feeding increased interleukin-1 beta and induced a similar pattern on interleukin-6 and TGF-beta, which were globally reduced by physical exercise. ET decreased HID leukemia inhibitory factor level, SD + VPA animals presenting higher values than HFD+VPA animals. HFD increased the ratio of IRS-1(Ser307)/total IRS-1, which was completely mitigated by physical exercise. Physical exercise reduced total ERK and JNK (total and activated) expression in HFD. In SD vs. HFD, VPA presented higher activated JNK and ET presented higher total JNK. Generally, in HFD, the ratio (activated/total) of Ala, and each separately, decreased with exercise and also for activated AKT in SD. Overall, in both diets, exercise reduced 11 beta-hydroxysteroid dehydrogenase type 1. ET increased glucocorticoid receptor and reduced PTP1B in HID. Significance: Physical exercise mitigates the expression of pro-inflammatory mediators and positively modulates insulin and glucocorticoid signaling in NASH.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10