Stem bark and flower extracts of Vismia cauliflora are highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Alessandra Braga Ribeiro (Author) Other; Alessandra Berto (Author) Other; Daniela Ribeiro (Author) Other; Marisa Freitas (Author) Other; Renan Campos Chiste (Author) Other; Jesui Vergilio Visentainer (Author) Other; Eduarda Fernandes (Author) FFUP

Journal

Title: Pharmaceutical Biology

Vol. 53

Pages: 1691-1698

ISSN: 1388-0209

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 8 Citations

Scientific classification

FOS: Engineering and technology > Medical engineering

Other information

Authenticus ID: P-00G-S29

DOI: 10.3109/13880209.2014.1001407

Abstract (EN): Context: Vismia cauliflora A. C. Sm. [Hypericaceae (Clusiaceae)] is an Amazonian plant traditionally used by indigenous population to treat dermatosis and inflammatory processes of the skin. Previous research on V. cauliflora extracts suggests its potential to neutralize cellular oxidative damages related to the production of reactive oxygen and nitrogen species. Objective: To determine the activity of stem bark and flower extracts of V. cauliflora on the modulation of oxidative burst in human neutrophils, as well as its potential to inhibit oxidative damage in human erythrocytes. Materials and methods: The modulation of neutrophil's oxidative burst by the ethanolic extracts (0.3-1000 mu g/mL) was determined by the oxidation of specific probes by reactive species. Additionally, the potential of these extracts to inhibit oxidative damage in human erythrocytes was evaluated by monitoring its biomarkers of oxidative stress. Results: Vismia cauliflora extracts presented remarkable capacity to prevent the oxidative burst in activated human neutrophils (IC50 < 15 mu g/mL). However, the maximum percentage of inhibition achieved against hydrogen peroxide was 45%. Concerning the oxidative damage in human erythrocytes, the extracts were able to minimize the tert-butyl hydroperoxide-induced hemoglobin oxidation and lipid peroxidation in a very low concentration range (2.7-18 mu g/mL). Furthermore, only stem bark extract (100 mu g/mL) was able to inhibit the depletion of glutathione (13%). Discussion and conclusion: These results reinforce the therapeutic potential of stem bark and flower extracts of V. cauliflora to heal topical skin disease, namely in the treatment of neutrophil-related dermatosis and skin conditions related to oxidative stress, including skin aging.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8