The effect of octadecyl chain immobilization on the hemocompatibility of poly (2-hydroxyethyl methacrylate)

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Marion Fischer (Author) Other; Catarina P Baptista (Author) Other; Ines C Goncalves (Author) FEUP; Buddy D Ratner (Author) Other; Claudia Sperling (Author) Other; Carsten Werner (Author) Other; Cristina L Martins (Author) Other; Mario A Barbosa (Author) FEUP

Journal

Title: Biomaterials

Vol. 33

Pages: 7677-7685

ISSN: 0142-9612

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 4 Citations

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

Other information

Authenticus ID: P-002-49D

DOI: 10.1016/j.biomaterials.2012.07.007

Abstract (EN): Albumin-scavenging surfaces decorated with n-alkyl chains represent an established strategy for blood-contacting applications. To evaluate this concept, a set of poly (2-hydroxyethyl methacrylate) (pHEMA) films modified with different amounts of octadecyl isocyanate (08) was investigated in an in vitro hemocompatibility assay using freshly drawn human whole blood. In addition, the hydrogel materials were thoroughly characterized with respect to changes in wettability and elasticity, which accompanied the gradual chemical modification of pHEMA. An increase of the surface C18 content induced enhanced hydrophobicity and stiffness. Immobilization of C18 chains was found to substantially reduce the coagulation activation and the complement activation by the pHEMA films. Platelet adhesion and degranulation (PF4 release) were similar on the modified and the unmodified pHEMA. Platelet adhesion to pHEMA hydrogels was lower than the polytetrafluoroethylene reference. We conclude that the immobilization of octadecyl chains improved the hemocompatibility of pHEMA materials under conditions that might be encountered in low shear blood flow.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9