In vitro analysis of protein adhesion to phase pure hydroxyapatite and silicon substituted hydroxyapatite

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Botelho, CM (Author) Other; Brooks, RA (Author) Other; Kawai, T (Author) Other; Ogata, S (Author) Other; Ohtsuki, C (Author) Other; Best, SM (Author) Other; Lopes, MA (Author) FEUP; Santos, JD (Author) FEUP; Rushton, N (Author) Other; Bonfield, W (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: BIOCERAMICS, VOL 17

Vol. 230-232

Pages: 461-464

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 21 Citations

Scientific classification

FOS: Engineering and technology > Materials engineering

Other information

Authenticus ID: P-000-58C

Abstract (EN): The adhesion of bovine collagen type I, bovine serum albumin, bovine IgG, 1% and 10% (v/v) human serum to hydroxyapatite (HA), silicon-substituted hydroxyapatite (Si-HA) and tissue culture plastic were studied. The materials were incubated at 37 degrees C for 30 minutes, after which the protein solution was removed and analyzed. The adsorbed protein was evaluated by electrophoresis and immunoassay after extraction from the materials. The degree of adhesion was higher for collagen, followed by IgG and albumin on all materials. However there was no difference in the amount of collagen adsorbed onto the surface of each material and this was also the finding with albumin and IgG. These results suggest that the increased bioactivity seen with Si-HA is not due to the degree of protein adhesion, but may possibly be due to changes in the conformation of the bound proteins.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4