Optimization of processing conditions for the quantification of enterococci biofilms using microtitre-plates

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Extremina, CI (Author) Other; Costa, L (Author) Other; Aguiar, AI (Author) Other; Peixe, L (Author) FFUP; Fonseca, AP (Author) Other

Journal

Title: Journal of Microbiological Methods

Vol. 84

Pages: 167-173

ISSN: 0167-7012

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 30 Citations

Scopus - 41 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-VA7

DOI: 10.1016/j.mimet.2010.11.007

Abstract (EN): We performed a comparison of four selected protocols from the literature using microtitre-plate and colorimetric biomass assay for evaluation of enterococci biofilm formation ability and optimized a protocol after the identification and stringent performance of biofilm formation steps. The optimized protocol uses a dynamic model that provided a greater discrimination of enterococci biofilm formation ability, and could better simulate in vivo real conditions. Moreover different biofilm quantification approaches, such as the colorimetric biomass (crystal violet), the resazurin and CFU's assays could be used with the optimized protocol, with adequate reproducibility. This study also recognizes that parameters such as the biofilm formation index (BFi), the cut-off values and the Z' factor provide greater accuracy, possibility of inter and intra laboratory comparison and quality evaluation of the biofilm screening assays, respectively.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7