A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Sonia Soares (Author) Other; Joana S Amaral (Author) Other; Beatriz B P P Oliveira (Author) FFUP; Isabel Mafra (Author) FFUP

Journal

Title: Meat Science

Vol. 94

Pages: 115-120

ISSN: 0309-1740

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 69 Citations

Scopus - 105 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-005-1F9

DOI: 10.1016/j.meatsci.2012.12.012

Abstract (EN): Species identification in meat products has grown in interest in recent years since these foodstuffs are susceptible targets for fraudulent labelling. In this work, a real-time PCR approach based on SYBR Green dye was proposed for the quantitative detection of pork meat in processed meat products. For the development of the method, binary meat mixtures containing known amounts of pork meat in poultry meat were used to obtain a normalised calibration model from 0.1 to 25% with high linear correlation and PCR efficiency. The method revealed high specificity by melting curve analysis, being successfully validated through its application to blind meat mixtures, which confirmed its adequacy for pork meat determination. The fully applicability of the method was further demonstrated in commercial meat products, allowing verification of labelling compliance and identification of meat species in processed foods.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6