Evaluation of the best method to assess antibiotic potentiation by phytochemicals against Staphylococcus aureus

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Ana Cristina Abreu (Author) FEUP; Sofia C. Serra (Author) Other; Anabela Borges (Author) FEUP; Maria José Saavedra (Author) Other; António J. Salgado (Author) Other; Manuel Simões (Author) FEUP

Journal

Title: Diagnostic Microbiology and Infectious Disease

Vol. 79 No. 2

Pages: 125-134

ISSN: 0732-8893

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 10 Citations

ISI Web of Science

Scopus - 17 Citations

Pubmed / Medline

Scientific classification

CORDIS: Health sciences

FOS: Engineering and technology

Associated Projects

Fitodesinfetantes - Desinfeção de bactérias planctónicas e biofilmes em ambientes hospitalares com produtos fitoquímicos

Bioresist - Estudo da influência do fenótipo de biofilmes na sua resiliência e resistência

Other information

Authenticus ID: P-009-HX9

DOI: 10.1016/j.diagmicrobio.2014.03.002

Abstract (EN): The increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 +/- 2.2 mg/L) and theophylline (IC50 = 100 +/- 4.7 mg/L).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10