Mineralization of 4-fluorocinnamic acid by a Rhodococcus strain

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Catarina L Amorim (Author) Other; Antonio C S Ferreira (Author) Other; Maria F Carvalho (Author) Other; Carlos M M Afonso (Author) FFUP; Paula M L Castro (Author) Other

Journal

Title: Applied Microbiology and Biotechnology

Vol. 98

Pages: 1893-1905

ISSN: 0175-7598

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 11 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-008-PJF

DOI: 10.1007/s00253-013-5149-6

Abstract (EN): A bacterial strain capable of aerobic degradation of 4-fluorocinnamic acid (4-FCA) as the sole source of carbon and energy was isolated from a biofilm reactor operating for the treatment of 2-fluorophenol. The organism, designated as strain S2, was identified by 16S rRNA gene analysis as a member of the genus Rhodococcus. Strain S2 was able to mineralize 4-FCA as sole carbon and energy source. In the presence of a conventional carbon source (sodium acetate [SA]), growth rate of strain S2 was enhanced from 0.04 to 0.14 h(-1) when the culture medium was fed with 0.5 mM of 4-FCA, and the time for complete removal of 4-FCA decreased from 216 to 50 h. When grown in SA-supplemented medium, 4-FCA concentrations up to 1 mM did not affect the length of the lag phase, and for 4-FCA concentrations up to 3 mM, strain S2 was able to completely remove the target fluorinated compound. 4-Fluorobenzoate (4-FBA) was transiently formed in the culture medium, reaching concentrations up to 1.7 mM when the cultures were supplemented with 3.5 mM of 4-FCA. Trans, trans-muconate was also transiently formed as a metabolic intermediate. Compounds with molecular mass compatible with 3-carboxymuconate and 3-oxoadipate were also detected in the culture medium. Strain S2 was able to mineralize a range of other haloorganic compounds, including 2-fluorophenol, to which the biofilm reactor had been exposed. To our knowledge, this is the first time that mineralization of 4-FCA as the sole carbon source by a single bacterial culture is reported.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13