Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

• Type: Article in International Scientific Journal
• Date: 2015

Authors

J. Barros (Author) Other; L. Grenho (Author) Other; M. H. Fernandes (Author) FMDUP; C. M. Manuel (Author) Other; L. F. Melo (Author) FEUP; O. C. Nunes (Author) FEUP; Fernando Mendes Monteiro (Author) FEUP; Ferraz, MP (Author) Other

Journal

Title: Colloids and Surfaces B: Biointerfaces

Vol. 130

Pages: 305-314

ISSN: 0927-7765

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 21 Citations

INSPEC

Scientific classification

FOS: Engineering and technology > Materials engineering

Associated Projects

NaNOBiofilm - NaNOBiofilm - Superfícies de nanohidroxiapatite com caracteristicas antiobacterianas para prevenção de infecção óssea associada a biofilmes

Other information

Authenticus ID: P-00G-5TT

DOI: 10.1016/j.colsurfb.2015.04.034

Abstract (EN): Nanohydroxyapatite possesses exceptional biocompatibility and bioactivity regarding bone cells and tissues, justifying its use as a coating material or as a bone substitute. Unfortunately, this feature may also encourage bacterial adhesion and biofilm formation. Surface functionalization with antimicrobials is a promising strategy to reduce the likelihood of bacterial infestation and colonization on medical devices. Chlorhexidine digluconate is a common and effective antimicrobial agent used for a wide range of medical applications. The purpose of this work was the development of a nanoHA biomaterial loaded with CHX to prevent surface bacterial accumulation and, simultaneously, with good cytocompatibility, for application in the medical field. CHX (5-1500 mg/L) was loaded onto nanoHA discs and the materials were evaluated for CHX adsorption and release profile, physic-chemical features, antibacterial activity against Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis, and cytocompatibility toward L929 fibroblasts. Results showed that the adsorption of CHX on nanoHA surface occurred by electrostatic interactions between the cationic group of CHX and the phosphate group of nanoHA. The release of CHX from CHX-loaded nanoHA showed a fast initial rate followed by a slower kinetics release, due to constraints caused by dilution and diffusion-limiting processes. NanoHA.50 to nanoHA.1500 showed strong anti-sessile activity, inhibiting bacterial adhesion and the biofilm formation. CHX-nanoHA caused a dose- and time-dependent inhibitory effect on the proliferation of fibroblasts for nanoHA.100 to nanoHA.1500. Cellular behavior on nanoHA.5 and nanoHA.50 was similar to control. Therefore, CHX-loaded nanoHA surfaces appear as a promising alternative to prevention of devices-related infections.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10