Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Azevedo, NF (Author) FEUP; Jardim, T (Author) Other; Almeida, C (Author) Other; Cerqueira, L. (Author) Other; Almeida, AJ (Author) Other; Rodrigues, F (Author) Other; Keevil, CW (Author) Other; Vieira, MJ (Author) Other

Journal

Title: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Vol. 100

Pages: 463-470

ISSN: 0003-6072

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 18 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-MA4

DOI: 10.1007/s10482-011-9595-9

Abstract (EN): Staphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as Staphylococcus aureus. The method was subsequently successfully adapted for the detection of S. epidermidis in mixed-species blood cultures both by microscopy and flow cytometry.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8