COMPARATIVE-STUDY OF DIFFERENT EXTRACTIVE PROCEDURES TO QUANTIFY MORPHINE IN URINE BY HPLC-UV

• Type: Article in International Scientific Journal
• Date: 1992

Authors

SOARES, ME (Author) Other; SEABRA, V (Author) Other; BASTOS, MDA (Author) FFUP

Journal

Title: Journal of Liquid Chromatography

Vol. 15

Pages: 1533-1541

ISSN: 0148-3919

Publisher: Marcel Dekker Inc.

Indexing

ISI Web of Knowledge - 26 Citations

Scopus - 22 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-Q3G

DOI: 10.1080/10826079208018306

Abstract (EN): A comparative study to extract and purify total morphine in urine is described using several procedure treatments of biological samples. Sep-Pak C18 cartridges, Extrelut columns and liquid-liquid conventional extraction with sequential purification through Extrelut column were assayed in order to establish the best procedure to quantitatively extract morphine from urine free of endogenous interferents. Analysis of the drug was made by HPLC-UV with a C18 column and methanol: acetate buffer (pH6.9) (70+30) as eluent. Recovery of morphine from spiked urine was 88.0% and 79.8% for concentrations of l-mu-g/ml and 5-mu-g/ml, respectively. The detection limit of the method was 0.2-mu-g/ml and coefficient of variation was 1.8% (n=4).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9