Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Eduarda Moita (Author) Other; Angel Gil Izquierdo (Author) Other; Carla Sousa (Author) FFUP; Federico Ferreres (Author) Other; Luis R Silva (Author) Other; Patricia Valentao (Author) FFUP; Raul Dominguez Perles (Author) Other; Nieves Baenas (Author) Other; Paula B Andrade (Author) FFUP

Journal

Title: PLoS ONE

Vol. 8 No. 1

Final page: e59131

ISSN: 1932-6203

Publisher: Public Library of Science

Indexing

ISI Web of Knowledge - 28 Citations

Scopus - 52 Citations

Other information

Authenticus ID: P-005-2HT

DOI: 10.1371/journal.pone.0059131

Abstract (EN): Oxidative stress and inflammation play important roles in disease development. This study intended to evaluate the anti-inflammatory and antioxidant potential of Echium plantagineum L. bee pollen to support its claimed health beneficial effects. The hydromethanol extract efficiently scavenged nitric oxide ((NO)-N-center dot) although against superoxide (O-2(center dot-)) it behaved as antioxidant at lower concentrations and as pro-oxidant at higher concentrations. The anti-inflammatory potential was evaluated in LPS-stimulated macrophages. The levels of (NO)-N-center dot and L-citrulline decreased for all extract concentrations tested, while the levels of prostaglandins, their metabolites and isoprostanes, evaluated by UPLC-MS, decreased with low extract concentrations. So, E. plantagineum bee pollen extract can exert anti-inflammatory activity by reducing (NO)-N-center dot and prostaglandins. The extract is able to scavenge the reactive species (NO)-N-center dot and O-2(center dot-) and reduce markers of oxidative stress in cells at low concentrations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11