SEQUENTIAL FLOW-INJECTION DETERMINATION OF IONIC AND TOTAL CALCIUM IN SALIVA

• Type: Article in International Scientific Journal
• Date: 1994

Authors

JUN, S (Author) Other; LIMA, JLFC (Author) FFUP; MONTENEGRO, MCBSM (Author) FFUP

Journal

Title: Journal of Trace Elements and Electrolytes in Health and Disease

Vol. 8

Pages: 93-98

ISSN: 0931-2838

Publisher: Elsevier B.V.

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-K0Q

Abstract (EN): In this paper a flow injection manifold for the sequential determination of ionic and total calcium in a small (75 mu L) sample of saliva is presented. This setup incorporates two detectors, a tubular potentiometric detector and an atomic absorption spectrophotometer, for determining the ionic and total calcium, respectively. Furthermore, the saliva samples can be injected directly into the manifold without any pre-treatment or loss of carbon dioxide. The results of the analyses of 20 saliva samples were in good agreement with those obtained by the two reference procedures, the direct potentiometry for ionic calcium and atomic absorption spectroscopy for total calcium. The paired Student's t-test showed that there were no statistical differences in the results obtained. The relative standard deviations of ten consecutive measurements of the same salive sample were approximately 3% for ionic calcium and 4% for total calcium. Effects of differences in coexisting ions, ionic strength, and pH between standard solutions and samples were negligible.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6