Expression and function of sodium transporters in two opossum kidney cell clonal sublines

• Type: Article in International Scientific Journal
• Date: 2002

Authors

gomes, p (Author) Other; xu, j (Author) Other; serrao, p (Author) Other; doria, s (Author) FMUP; jose, pa (Author) Other; soares-da-silva, p (Author) FMUP

Journal

Title: American Journal of Physiology - Renal Fluid and Electrolyte Physiology

Vol. 283

Pages: F73-F85

ISSN: 0363-6127

Publisher: American Physiological Society

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 13 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-000-NR5

DOI: 10.1152/ajprenal.00340.2001

Abstract (EN): The present study describes characteristic features of two clonal subpopulations of opossum kidney (OK) cells (OK(LC) and OK(HC)) that are functionally different but morphologically identical. The most impressive differences between OK(HC) and OK(LC) cells are the overexpression of Na(+)-K(+)-ATPase and type 3 Na(+)/H(+) exchanger by the former, accompanied by an increased Na(+)-K(+)-ATPase activity (57.6+/-5.6 vs. 30.0+/-0.1 nmol P(i).mg protein(-1).min(-1)); the increased ability to translocate Na(+) from the apical to the basolateral surface; and the increased Na(+)-dependent pH(i) recovery (0.254+/-0.016 vs. 0.094+/-0.011 pH units/s). V(max) values (in pH units/s) for Na(+)-dependent pH(i) recovery in OK(HC) cells (0.00521+/-0.0004) were twice (P<0.05) those in OK(LC) (0.00202 +/- 0.0001), with similar K(m) values (in mM) for Na(+) (OK(LC), 21.0 +/- 5.5; OK(HC), 14.0 +/- 5.6). In addition, we measured the activities of transporters (organic ions, alpha-methyl-D-glucoside, L-type amino acids, and Na(+)) and enzymes (adenylyl cyclase, aromatic L-amino acid decarboxylase, and catechol-O-methyltransferase). The cells were also characterized morphologically by optical and scanning electron microscopy and karyotyped. It is suggested that OK(LC) and OK(HC) cells constitute an interesting cell model for the study of renal epithelial physiology and pathophysiology, namely, hypertension.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13