Manufacture of bioactive peptide-rich concentrates from Whey: Characterization of pilot process

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Tavares, T. (Author) FEUP; Manuela Amorim (Author) Other; David Gomes (Author) Other; Manuela E. Pintado (Author) Other; Carlos D. Pereira (Author) Other; F. Xavier Malcata (Author) FEUP

Journal

Title: Journal of Food Engineering

Vol. 110 No. 4

Pages: 547-552

ISSN: 0260-8774

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 21 Citations

ISI Web of Science

Scopus - 27 Citations

Scientific classification

FOS: Engineering and technology

Other information

Authenticus ID: P-002-9X1

DOI: 10.1016/j.jfoodeng.2012.01.009

Abstract (EN): This work was focused on the manufacture, at pilot scale, of cow whey protein and peptide concentrates, using selective filtration techniques-associated with hydrolysis brought about by proteolytic enzymes from Cynara cardunculus aqueous extracts, using as (optimal) conditions an enzyme/substrate ratio of 1.6% v/v, a pH of 5.2, a temperature of 55 degrees C and an incubation time of 7 h. The profiles of proteins and peptides were assessed by liquid chromatography and electrophoresis; ca. 87% of alpha-lactalbumin was hydrolyzed, but essentially no degradation of beta-lactoglobulin (beta-Lg) was observed. A bioactive peptide concentrate, its fraction below 3 kDa and a beta-Lg-rich fraction were obtained as final products, containing ca. 73, 43 and 91% w/w protein (on a total solid mass basis). All these fractions were low in lactose and salt, and their microbial loads were reduced. Said fractions are high added-value products, so they can be used as nutritional and functional ingredients-thus yielding an economically viable alternative for upgrade of whey.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6