Biochemistry

Code:

103

Acronym:

103

Instance: 2004/2005 - A

Active?	Yes
Web Page:	http://medicina.med.up.pt/bioquimica/
Course/CS Responsible:

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MD	102	Plano oficial a partir de 2000	1	10	17,5	-

Objectives

Acquisition of the specific language of Biochemistry; Knowledge of its study methods and of fundamental laws and contemporary theories in this scientific area; Knowledge of characteristic chemical composition of living organisms, of biological systems essential dynamic aspects, of molecular physiology mechanisms, and of fundamental biochemical concepts essential for understanding the human being, both in health and in disease.

Program

Chemical components of living organisms; peculiarities; typical components, Minerals. Water. Chemical reactions; chemical structures and phase systems. Isomery. Oxidation-reduction. Bioenergetics. Energy in biological systems. Calorimetry; basal metabolism and metabolism under several activity conditions. Biological energy accumulators. Cycle of ATP formation and utilization. Biochemical kinetics. Enzimes; kinetics of enzyme reactions. Enzime inhibitors and effectors. Enzyme classification; kinases and phosphatases. Carbohydrates; carbohydrate metabolism. Lipids; lipid metabolism. Tricarboxylic acid cycle. Proteins; protein metabolism. Protein glycosylation. Extracellular matrix biochemistry. Nucleic Acids. Genetic information and protein synthesis. Purine and pyrimidine nucleotide metabolism. Vitamins and co-enzimes. Integration of metabolism. Levels of homeostasis. Genetics. Membranes and biochemical transport. Acid-base balance. Osmotic balance. Correlation mechanisms (neurocrine, endocrine, paracrine).Immunology. Nutritional balance. Organization of biological systems. Levels of complexity. Ontogenesis. Filogenesis. Carcinogenesis. Biological species. Interspecific associations. Biochemistry and ecology..
This program will be developed in lectures, seminars and small group classes. In practical laboratorial sessions experimental protocols will be executed, allowing the learning of instrumental methods of analysis (spectrophotometry, potenciometry, flame photometry and polarimetry) and the effective analysis or characterization of a series of products (anions, cations, glycerol, carbohydrates, sucrose, starch, lipids including the steroids, proteins, enzymes). Amplification and semi-quantification of RNAm by PCR (polymerase chain reaction) will be proposed.

Main Bibliography

- Berg J M, Tymoczko J L, Stryer L: Biochemistry . 5Th Ed, International Edition.W H Freeman and Company. New York, 2002
- Murray, R.K., Granner, D.K., Mayes, P.A. & Rodwell, V.W.. Harper’s Biochemistry. Prentice-Hall. International Inc. London, 2000.
- Practices of Biochemistry for the Sciences of the Health Hipólito-Reis C, Raised M N, Azevedo I. Lidel, 2002 .
-Principles of Medical Biochemistry. Meisenberg G, Simmons W H. Mosby, 1998
-Biochemistry. An Introduction. Mckee T, Mckee J R. Wm. C. Brown Publishers, London, 1996.
-Molecular Biology of the Cell. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson J D. 4th Ed, Garland Publishing Inc, New York & London, 2001;
-Chemistry (Portuguese edition). Chang R: McGraw-Hill, Lisbon, 1994.

Teaching methods and learning activities

- Prelections on Monday and Wednesday from 12.00 to 13.00.
- Seminars on Wednesdays at 11.00.
- Practical works and group works of 2.30 each, according to the timetable of each class.

Evaluation Type

Distributed evaluation with final exam

Observations

Teaching staff:
Regent of the discipline: Prof. Doutora Isabel Azevedo
Teachers: Prof. Doutora Isabel Azevedo(Professor); Doutora Maria de Fátima Moreira Martel; Doutor Manuel Nuno Magalhães Pinheiro Alçada; Drª. Maria João Reis Conceição Martins (Auxiliary Teachers); Dr. João Tiago Sousa Pinto Guimarães Invited Auxiliary Teacher, 40%); Dr. Maria Rita Baldaque Sousa Soares Silva Negrão (Assistant); Dr. Raquel Ângela Silva Soares Lino and Dr. Maria Cristina Ramos Machado Lopes Abreu (Invited Assistants, 40%); Drª Elisa Keating, Dr. Lina Isabel Melão, Dr. Tiago Gregório and Dr. Duarte Torres (Monitors.