Go to:
Logótipo
Você está em: Start > Publications > View > Transcriptional regulation of the human mitochondrial peptide deformylase (PDF)
Map of Premises
Principal
Publication

Transcriptional regulation of the human mitochondrial peptide deformylase (PDF)

Title
Transcriptional regulation of the human mitochondrial peptide deformylase (PDF)
Type
Article in International Scientific Journal
Year
2012
Authors
Isabel Pereira Castro
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. Without AUTHENTICUS Without ORCID
Luis Teixeira da Costa
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. View Authenticus page Without ORCID
Antonio Amorim
(Author)
FCUP
View Personal Page You do not have permissions to view the institutional email. Search for Participant Publications View Authenticus page View ORCID page
Luisa Azevedo
(Author)
FCUP
View Personal Page You do not have permissions to view the institutional email. Search for Participant Publications View Authenticus page View ORCID page
Journal
Vol. 421
Pages: 825-831
ISSN: 0006-291X
Publisher: Elsevier
Scientific classification
FOS: Natural sciences > Biological sciences
Other information
Authenticus ID: P-002-A07
Abstract (EN): The last years of research have been particularly dynamic in establishing the importance of peptide deformylase (PDF), a protein of the N-terminal methionine excision (NME) pathway that removes formylmethionine from mitochondrial-encoded proteins. The genomic sequence of the human PDF gene is shared with the COG8 gene, which encodes a component of the oligomeric golgi complex, a very unusual case in Eukaryotic genomes. Since PDF is crucial in maintaining mitochondrial function and given the atypical short distance between the end of COG8 coding sequence and the PDF initiation codon, we investigated whether the regulation of the human PDF is affected by the COG8 overlapping partner. Our data reveals that PDF has several transcription start sites, the most important of which only 18 bp from the initiation codon. Furthermore, luciferase-activation assays using differently-sized fragments defined a 97 bp minimal promoter region for human PDF, which is capable of very strong transcriptional activity. This fragment contains a potential Sp1 binding site highly conserved in mammalian species. We show that this binding site, whose mutation significantly reduces transcription activation, is a target for the Sp1 transcription factor, and possibly of other members of the Sp family. Importantly, the entire minimal promoter region is located after the end of COG8's coding region, strongly suggesting that the human PDF preserves an independent regulation from its overlapping partner.
Language: English
Type (Professor's evaluation): Scientific
Contact: isabelpereiracastro@gmail.com; lazevedo@ipatimup.pt
No. of pages: 7
Documents
We could not find any documents associated to the publication.
Related Publications

Of the same journal

Human carbamoyl phosphate synthetase I (CPSI): Insights on the structural role of the unknown function domains (2012)
Another Publication in an International Scientific Journal
Monica Lopes Marques; Gilberto Igrejas; Antonio Amorim; Luisa Azevedo
Unusual self-association properties of transthyretin Y114C related to familial amyloidotic polyneuropathy: Effects on detection and quantification (1999)
Article in International Scientific Journal
Ando, Y; Almeida, MD; Ohlsson, PI; Ando, E; Negi, A; Suhr, O; Terazaki, H; Obayashi, K; Ando, M; Saraiva, MJM
TP53 and P21 polymorphisms: Response to cisplatinum/paclitaxel-based chemotherapy in ovarian cancer (2006)
Article in International Scientific Journal
Santos, AM; Sousa, H; Portela, C; Pereira, D; Pinto, D; Catarino, R; Rodrigues, C; Araujo, AP; Lopes, C; Medeiros, R
The influence of HER2 genotypes as molecular markers in ovarian cancer outcome (2005)
Article in International Scientific Journal
Pinto, D; Pereira, D; Portela, C; da Silva, JL; Carlos, LA; Medeiros, R

See all (23)

Recommend this page Top
Copyright 1996-2025 © Faculdade de Medicina Dentária da Universidade do Porto  I Terms and Conditions  I Acessibility  I Index A-Z  I Guest Book
Page created on: 2025-06-23 at 14:20:58 | Acceptable Use Policy | Data Protection Policy | Complaint Portal