Evidence of Browning and Inflammation Features in Visceral Adipose Tissue of Women with Endometriosis

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Abobeleira, JP (Author) Other; Neto, AC (Author) Other; Mauersberger, J (Author) Other; Salazar, M (Author) Other; Botelho, M (Author) Other; Fernandes, AS (Author) Other; Martinho, M (Author) Other; Maria Paula Serrão (Author) FMUP; Rodrigues, AR (Author) Other; Almeida, H (Author) Other; Gouveia, Alexandra (Author) FMUP; D Neves (Author) FMUP

Journal

Title: Archives of Medical Research

ISSN: 0188-4409

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 1 Citation

Other information

Authenticus ID: P-016-Y2F

DOI: 10.1016/j.arcmed.2024.103064

Abstract (EN): Background: Patients with endometriosis tend to have a low body mass index, suggesting an inverse relationship between body fat and risk of disease. This is supported by evidence that miRNAs differentially expressed in endometriosis induce browning of pre-adipocytes in vitro. Thus, we hypothesize that endometriosis may underlie adipose tissue (AT) dysfunction and browning. Aims: Identify inflammation and browning processes in AT collected from endometriosis patients. Methods: Visceral and subcutaneous AT samples were obtained during endometriosis (n = 32) or uterine myoma (n = 14; controls) surgery. Blood catecholamines were determined by high-performance liquid chromatography while IL-6 and TGF-beta levels were quantified by ELISA. Adipocyte cross-sectional areas were analyzed in H&E-stained sections by computer-assisted morphometry. Macrophages (F4/80; Galectin-3) and browning activation (UCP-1; PGC-1 alpha) in tissues were identified by dual label immunofluorescence. Expression of inflammatory (IL-6; MCP-1; Galectin-3; CD206; TIMP1; TGF-beta) and browning-related (UCP-1; PGC-1 alpha; DIO2; CITED1; CIDEA; TMEM26; TBX1; PRDM16; PPAR-gamma) molecules in AT were assessed by RT-PCR and Western blotting. Results: Compared to controls, patients presented smaller adipocytes, especially in VAT, and lower norepinephrine levels. Serum IL-6, but not TGF-beta, was increased in patients. UCP-1, PGC-1 alpha, IL-6, and MCP-1 were upregulated in VAT from endometriosis women, which also evidenced a reduction of CD206, relative to controls. However, no differences were found in mRNA expression of IL-6, TIMP1, and TGF-beta nor Galectin-3 protein levels. In SAT, protein expression remained unchanged between patients and controls. Conclusions: Our findings support an endometriosis' role as a pro-catabolic state along with local signals of VAT browning and inflammation. (c) 2024 The Author(s). Published by Elsevier Inc. on behalf of Instituto Mexicano del Seguro Social (IMSS). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

Language: English

Type (Professor's evaluation): Scientific