Modeling the fluid-dynamics and oxygen consumption in a porous scaffold stimulated by cyclic squeeze pressure

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Ferroni, M (Author) Other; Giusti, S (Author) Other; Nascimento, D (Author) ICBAS; Silva, A (Author) Other; Boschetti, F (Author) Other; Ahluwalia, A (Author) Other

Journal

Title: Medical Engineering & Physics

Vol. 38

Pages: 725-732

ISSN: 1350-4533

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-00K-RCR

DOI: 10.1016/j.medengphy.2016.04.016

Abstract (EN): The architecture and dynamic physical environment of tissues can be recreated in-vitro by combining 3D porous scaffolds and bioreactors able to apply controlled mechanical stimuli on cells. In such systems, the entity of the stimuli and the distribution of nutrients within the engineered construct depend on the micro-structure of the scaffolds. In this work, we present a new approach for optimizing computational fluid-dynamics (CFD) models for the investigation of fluid-induced forces generated by cyclic squeeze pressure within a porous construct, coupled with oxygen consumption of cardiomyocytes. A 2D axial symmetric macro-scaled model of a squeeze pressure bioreactor chamber was used as starting point for generating time dependent pressure profiles. Subsequently the fluid movement generated by the pressure fields was coupled with a complete 3D micro-scaled model of a porous protein cryogel. Oxygen transport and consumption inside the scaffold was evaluated considering a homogeneous distribution of cardiomyocytes throughout the structure, as confirmed by preliminary cell culture experiments. The results show that a 3D description of the system, coupling a porous geometry and time dependent pressure driven flow with fluid structure-interaction provides an accurate and meaningful description of the microenvironment in terms of shear stress and oxygen distribution than simple stationary 2D models.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8