MALDI-TOF MS and chemometric based identification of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex species

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Sousa, C (Author) Other; Botelho, J (Author) Other; Silva, L (Author) Other; Grosso, F (Author) Other; Nemec, A (Author) Other; Lopes, J (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: International Journal of Medical Microbiology

Vol. 304

Pages: 669-677

ISSN: 1438-4221

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 37 Citations

Scopus - 44 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-009-PC6

DOI: 10.1016/j.ijmm.2014.04.014

Abstract (EN): MALDI-TOF MS is becoming the technique of choice for rapid bacterial identification at species level in routine diagnostics. However, some drawbacks concerning the identification of closely related species such as those belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (Acb) complex lead to high rates of misidentifications. In this work we successfully developed an approach that combines MALDI-TOF MS and chemometric tools to discriminate the six Acb complex species (A. baumannii, Acinetobacter nosocomialis, Acinetobacter pittii, A. calcoaceticus, genomic species "Close to 13TU" and genomic species "Between 1 and 3"). Mass spectra of 83 taxonomically well characterized clinical strains, reflecting the breadth of currently known phenetic diversity within the Acb complex, were achieved from intact cells and cell extracts and analyzed with hierarchical cluster analysis (HCA) and partial least squares discriminant analysis (PLSDA). This combined approach lead to 100% of correct species identification using mass spectra obtained from intact cells. Moreover, it was possible to discriminate two Acb complex species (genomic species "Close to 13TU" and genomic species "Between 1 and 3") not included in the MALDI Biotyper database.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9