Valorization of Kiwiberry Leaves Recovered by Ultrasound-Assisted Extraction for Skin Application: A Response Surface Methodology Approach

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Silva, AM (Autor) Outra; Pinto, D (Autor) Outra; Moreira, MM (Autor) Outra; Paulo Costa (Autor) FFUP; Delerue-Matos, C (Autor) Outra; Rodrigues, F (Autor) Outra

Revista

Título: Antioxidants

Vol. 396

Página Final: 763

ISSN: 2076-3921

Editora: MDPI

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00W-FCW

DOI: 10.3390/antiox11040763

Abstract (EN): This study aims to evaluate the optimal ultrasound-assisted extraction (UAE) conditions of antioxidants polyphenols from Actinidia arguta (Siebold & Zucc.) Planch. Ex Miq. (kiwiberry) leaves using a response surface methodology (RSM). The effects of solid:liquid ratio (2.5-10.0% w/v), time (20-60 min), and intensity (30-70 W/m(2)) on the total phenolic content (TPC) and antioxidant/antiradical activities were investigated. The optimal UAE conditions were achieved using a solid:liquid ratio of 10% (w/v) and an ultrasonic intensity of 30 W/m(2) for 31.11 min. The results demonstrated that the optimal extract showed a high TPC (97.50 mg of gallic acid equivalents (GAE)/g dw) and antioxidant/antiradical activity (IC50 = 249.46 mu g/mL for ABTS assay; IC50 = 547.34 mu g/mL for DPPH assay; 1440.13 mu mol of ferrous sulfate equivalents (FSE)/g dw for ferric reducing antioxidant power (FRAP)) as well as a good capacity to scavenge superoxide and hypochlorous acid (respectively, IC50 = 220.13 mu g/mL and IC50 =10.26 mu g/mL), which may be related with the 28 phenolic compounds quantified. The in vitro cell assay demonstrated that the optimal extract did not decrease the keratinocytes' (HaCaT) viability, while the fibroblasts' (HFF-1) viability was greater than 70.63% (1000 mu g/mL). This study emphasizes the great potential of kiwiberry leaves extracted by UAE for skin application.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15