Optimization of culture conditions to improve helicobacter pylori growth in ham's F-12 medium by response surface methodology

• Type: Article in International Scientific Journal
• Date: 2012

Authors

L. J. Bessa (Author) Other; D. M. Correia (Author) Other; L. Cellini (Author) Other; N. F. Azevedo (Author) FEUP; I. Rocha (Author) Other

Journal

Title: International Journal of Immunopathology and Pharmacology

Vol. 25 No. 4

Pages: 901-909

ISSN: 0394-6320

Publisher: SAGE

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Associated Projects

PEst-C/EQB/UI0511/2011 - PROJECTO ESTRATÉGICO - UI 511 - 2011-2012

Other information

Authenticus ID: P-002-4J0

DOI: 10.1177/039463201202500408

Abstract (EN): Helicobacter pylori is a gastroduodenal pathogen that colonizes the human stomach and is the causal agent of gastric diseases. From the clinical and epidemiological point of view, enhancing and improving the growth of this bacterium in liquid media is an important goal to achieve in order to allow the performance of accurate physiological studies. The aim of this work was to optimize three culture conditions that influence the growth of H. pylori in the defined medium Ham's F-12 supplemented with 5% fetal bovine serum by using response surface methodology as a statistical technique to obtain the optimal conditions. The factors studied in this experimental design (Box-Behnken design) were the pH of the medium, the shaking speed (rpm) and the percentage of atmospheric oxygen, in a total of 17 experiments. The biomass specific growth rate (mu) was the response measured. The model was validated for pH and shaking speed. The percentage of atmospheric oxygen did not influence the growth for the range of values studied. At the optimal values found for pH and shaking speed, 8 and 130 rpm, respectively, a specific growth rate value of 0.164 h(-1), corresponding to a maximal concentration of approximately 1.5x10(8) CFU/ml, was reached after 8 h. The experimental design strategy allowed, for the first time, the optimization of H. pylori growth in a semi-synthetic medium, which may be important to improve physiological and metabolic studies of this "fastidious" bacterium.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9