Polyunsaturated fatty acids production by solid-state fermentation on polyurethane foam by Mortierella alpina

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Ferreira, M (Author) Other; Fernandes, H (Author) Other; Peres, H (Author) FCUP; Oliva Teles, A (Author) FCUP; Belo, I (Author) Other; Salgado, JM (Author) Other

Journal

Title: Biotechnology Progress

Vol. 37

ISSN: 8756-7938

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00T-AMV

DOI: 10.1002/btpr.3113

Abstract (EN): Polyunsaturated fatty acids (PUFAs) are essential in healthy diets and their production is extremely important. Natural sources of PUFAs includes animal and aquatic products such as marine fish oil, however there are several limitations such as the decrease of fish stocks throughout the world. Thus, microbial oils are a preferable source of PUFAs. Herein, it was studied the production of PUFAs by Mortierella alpina under solid-state fermentation (SSF) using polyurethane foam as inert substrate and synthetic medium or lignocellulosic hydrolysate as source of C, N, and other nutrients. Several parameters of fermentation conditions were evaluated as carbon source, inductors addition, ratio C/N and temperature. The highest amount of total PUFAs per mass of solid (535.41 +/- 24.12 mg/g), linoleic acid (129.66 +/- 5.84 mg/g), and alpha-linoleic acid (401.93 +/- 18.10 mg/g) were produced when the culture medium contained 20 g/L glucose, 10% (w/v) linseed oil, the C/N ratio was adjusted to 25 and the incubation temperature was 25 degrees C for 3 days decreasing to 16 degrees C on the remaining 4 days of fermentation. In addition, a hemicellulosic hydrolysate can be used as low-cost substrate to produce PUFAs, although the production was lower than the achieved with synthetic medium. SSF showed an interesting technology for microbial PUFAs production.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12