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link to PhD Thesis (18351-bromatology)










































































Food Authenticity

Food authenticity using PCR-based molecular markers
  • Quantitative detection of cow’s milk in ewes’ and goats’ milk cheeses by duplex polymerase chain reaction

  • Development of DNA markers – genomic or mithocondrial – for olive oil authenticity evaluation

  • Development and evaluation of DNA extraction techniques from recalcitrant matrices

  • Varietal discrimination using DNA markers – SSRs and ITS – for the populations characterization

  • Detection of GMO

    Relevant publications:

    A duplex polymerase chain reaction for the quantitative detection of cow’s milk in goat’s cheese.
    I. Mafra, A. Roxo, I.M.P.L.V.O. Ferreira, M.B.P.P. Oliveira.
    Int. Dairy J, 17, 1132-1138, 2007

    Grapevine clones discriminated with Stilbene Synthase –Chalcone Synthase markers.
    M. A. Faria, M. Beja-Pereira, A. Martins , M. A. Ferreira, M. E. S. Nunes.
    J. Sci. Food Agric., 84 1186-1192, 2005

    A novel approach to the quantification of bovine milk in ovine cheeses using a duplex polymerase chain reaction method.
    I. Mafra, I.M.P.L.V.O. Ferreira, Miguel A. Faria, Beatriz P. P. Oliveira.
    J. Agric. Food Chem., 52, 4943-4947, 2004

    Verifying the identity and parentage of Cruzado de Rabo de Ovelha with microsatellite markers.
    R.Magalhães, M. A.Faria, N. M. M. Santos, J. E. E. Dias, N. Magalhães, C. P.Meredith, F. F. Monteiro.
    Am. J. Enol. Vitic., 54 56-58, 2003

    Vitis vinifera grape must varietal authentication using microsatellite DNA analysis.
    M. A. Faria, R. Magalhães, M. A. Ferreira, C. P. Meredith, F. Ferreira-Monteiro.
    J. Agric. Food Chem., 48 1096-1100, 2000



Food authenticity using chemical markers: Major and minor food components are used as chemical markers for food authenticity

    Relevant publications:

    Discrimination of vegetable oils by triacylglycerols evaluation of profile using HPLC/ELSD.
    S.C. Cunha, M.B.P.P. Oliveira.
    Food Chemistry, 95, 518–524, 2006

    Discrimination between Arabica and Robusta Coffee Species on the Basis of their amino acid enantiomers.
    S. Casal, M.R. Alves, E. Mendes, M.B.P.P. Oliveira, M.A. Ferreira.
    Journal of Agriculture and Food Chemistry, 51, 6495-6501, 2003

    Detection and quantification of bovine, ovine and caprine milk percentages in Protected Denomination of Origin Cheeses by reversed-phase high-performance liquid chromatography of beta-lactoglobulins.
    I.M.P.L.V.O. Ferreira, H. Caçote.
    J. Chromatog. A, 1115, 111-118, 2003

    Discrimination between Arabica and Robusta Coffee Species on the Basis of their amino acid enantiomers.
    S. Casal, M. R. Alves, E. Mendes, M. B. P.P. Oliveira, M. A. Ferreira
    Journal of Agriculture and Food Chemistry, 51, 6495-6501, 2003



Bioinformatics applied to the development of new authenticity markers
    Relevant publications:

    Classification of PDO olive oils on the basis of their sterol composition by multivariate analysis.
    M. Rui Alves, S.C. Cunha, J.S. Amaral, J.A. Pereira, M.B.P.P. Oliveira.
    Analytica Chimica Acta, 549, 166-178, 2005


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