Analytical Methods in Toxicology

Code:

TA07008

Acronym:

MAT

Keywords
Classification	Keyword
OFICIAL	Physical Sciences

Instance: 2022/2023 - SP

Active?	Yes
Web Page:	https://moodle.up.pt/course/view.php?id=1231#section-2
Responsible unit:	Applied Chemistry Laboratory
Course/CS Responsible:	MSc in Analytical, Clinical and Forensic Toxicology

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
TACF	18	Official Curriculum	1	-	9	81	243

Teaching language

Suitable for English-speaking students
Obs.: /também falantes de francês, castelhano e italiano)

Objectives

Globally (i.e., with the theoretical and laboratory classes) it is intended to develop in the students the ability to apply the instrumental methods of analysis in real situations and the acquisition of skills as laboratory analysts. Specifically it is intended that students become able to:
i) Recognize the importance of instrumental methods of analysis; ii) know the main instrumental techniques and the basics of instrumentation; iii) Perform the development and validation of analytical procedures; iv) Perform the proper treatment of laboratory results.

Learning outcomes and competences

The UC contents aim to ensure the learning objectives/skills acquisition. Actually, the proper use of instrumental methods of analysis in the laboratory requires an adequate theoretical and practical knowledge of: i) the techniques fundamentals and the analytical instrumentation operation basics; ii) the appropriate validation of the analytical procedures; iii) the pre-analytical samples processing; iv) the proper treatment of laboratory data.
Aspects relating to the analytical quality control and automation of analytical procedures will also be addressed. All integrated, it can assure the training of students to work effectively and efficiently at the laboratory setting.

Working method

Presencial

Pre-requirements (prior knowledge) and co-requirements (common knowledge)

Proper knowledge of analytical chemistry, organic and inorganic chemistry, physiscs and statistics basic concepts are required for full comprehension of the different course issues. Classes are given in Portuguese language. Recommend books and most materials are in English.

Program

I. BASICS
I.1. Basic tools and concepts
I.2. Analytical Methods: selection, development and validation
I.3. Evaluation of analytical data – basic statistical parameters
II. ATOMIC SPECTROSCOPY
II.1. Introduction
II.2. Components of instruments
II.3. Atomic absorption
II.4. Atomic emission
II.5. Atomic mass spectrometry
III. MOLECULAR SPECTROSCOPY
III.1. UV/Vis absorption spectroscopy
III.2. IR spectroscopy
III.3. Fluorimetry and chemiluminescence
IV. ELECTROANALYTICAL METHODS
IV.1. Potentiometric methods
IV.2. Voltammetric and coulometric methods
IV.3. Conductometry
V. SEPARATION METHODS
V.1. General aspects
V.2. Gas chromatography
V.3. High-performance liquid chromatography
V.4. Other chromatographic / separation techniques
VI. OTHER TOPICS
VI.1. (Molecular) Mass spectrometry
VI.2. Turbidimetry and Nephelometry
VI.3. Particle size analysis
VI.4. Automated methods of analysis
VI.5. Sample preparation

Mandatory literature

Skoog Douglas A.; Principles of instrumental analysis. ISBN: 0-03-001229-5
Rouessac Francis; Chemical analysis. ISBN: 978-0-470-85903-2
Harris Daniel C.; Quantitative chemical analysis. ISBN: 0-7167-4464-3

Teaching methods and learning activities

Tutorial lectures provide the proper theoretical background. Laboratory classes allow students to apply Instrumental Methods of Analysis to real situations and the acquisition of skills as analysts.

Evaluation Type

Distributed evaluation with final exam

Assessment Components

designation	Weight (%)
Exame	80,00
Trabalho laboratorial	20,00
Total:	100,00

Amount of time allocated to each course unit

designation	Time (hours)
Estudo autónomo	0,00
Frequência das aulas	0,00
Trabalho laboratorial	30,00
Total:	30,00

Eligibility for exams

Student's attendance to the laboratorial classes is mandatory, according to General Evaluation Rules in force at FFUP. 
Approval requires laboratory assessment of > 10 points.

Calculation formula of final grade

Final classification (0-20): Distributed evaluation (20%) + final exam (80%)

Notes:
1. Distributed evaluation = performance in laboratory classes + reports of the laboratory work.
2. The final exam is a written test. Students who obtain a final classificação between 8 and 9.5 will be submitted to an oral exam.
3. Students who by law are exempt from the attendance to laboratory classes will be submitted to a laboratory exam.

Examinations or Special Assignments

not applied

Special assessment (TE, DA, ...)

Students who by law are exempt from the attendance to laboratory classes will be submitted to a laboratory exam.

Classification improvement

According to "Normas Gerais de de Avaliação" in force at FFUP.

Observations

Changes arising from the Covid-19 Contingency Plan


Scenario of limitation in the capacity of classrooms for face-to-face classes

1. The timetable for classes and distributed assessment is adjusted.
2. Changes to “Objectives”, “Learning Outcomes and Competences” and “program”: 

• No substantial change

3. Changes to “Teaching methods and learning activities”:

• Theoretical classes will be in person, limited to the number dictated by the room capacity, with synchronous videoconference, at the established times and using platforms such as Colibri-Zoom and Panopto for students who do not have access to the room.


• The laboratory classes take place in the laboratories, respecting 50% of the usual capacity, with the division of the class in odd and even shifts.

4. Changes in “Type of Assessment”, “Assessment Component”, “Occupation Component”; Frequency Obtaining ”; “Formula for calculating the final classification” and “Classification Improvement”: 

• No substantial changes, however, some evaluation processes may take place at a distance.

Scenario of all classes taking place at distance

1. The timetable for classes and distributed assessment is adjusted.
2. Changes to “Objectives”, “Learning Outcomes and Skills” and “program”: 

• No substantial changes, except that there will be no practical laboratory demonstration component.

3. Changes to “Teaching methods and learning activities”:

• Classes will be taught in synchronous videoconference, at the established times and using platforms such as Colibri-Zoom and Panopto.

4. Changes in “Type of Assessment”, “Assessment Component”, “Occupation Component”; Frequency Obtaining ”; “Formula for calculating the final classification” and “Classification Improvement”:

• No substantial changes, however, some evaluation processes must take place at a distance.